The article refers to an experiment conducted by a group of 9 scientists at the Institute of Cancer Research in London who wished to develop a mouse model of melanomas driven by the BRAF gene. This model was accomplished and can be used as a comparative for human genes as the melanomas which were developed by the mice have similarities to those found in humans. What the research found was that the expression of BRAFV600E stimulates skin hyperpigmentation and nevi harbouring which produce melanomas. Thus the BRAF gene has been shown to be essential for melanoma growth and maintenance in humans. The experiments showed that the BRAF gene stimulated several of the factors for the progression of tumours such as cell signalling, growth and transformation. Although the study proved that BRAFV600E was a key factor for the production of the melanoma’s the data indicated that the gene alone does not create the disease. It is unsure whether it is a driver, founder or passenger mutagenic.
The experiment was conducted using the DNA/RNA techniques of RT-PCR, to create the specific genes necessary for the continuing trials and by using DNeasy and RNeasy kits to extract the DNA and RNA from frozen tissues and cells.
The full article can be viewed here: http://www.cell.com/cancer-cell/fulltext/S1535-6108(09)00074-9
The experiment was conducted using the DNA/RNA techniques of RT-PCR, to create the specific genes necessary for the continuing trials and by using DNeasy and RNeasy kits to extract the DNA and RNA from frozen tissues and cells.
The full article can be viewed here: http://www.cell.com/cancer-cell/fulltext/S1535-6108(09)00074-9
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